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Item #:
68232459
50X TAE Buffer (Tris-Acetate-EDTA), 500 mL
Item #:
68232459
TJS 652
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50X TAE Buffer is used for running electrophoresis of nucleic acids in the gels, providing electrical conductivity and maintaining the pH.
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What Stands Out
Optimal pH Stability
This 50X TAE buffer ensures stable pH levels vital for consistent DNA and RNA electrophoresis, enhancing accuracy and reliability in experiments.
Efficient Concentration
Highly concentrated at 50X, this buffer allows for easy dilution, saving time and reducing storage space while providing sufficient supplies for multiple applications.
Quality Assured
Manufactured with high-quality chemicals, this TAE buffer guarantees purity and performance, ensuring reliable results for researchers and laboratories focusing on molecular biology.
Product Details
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use.
- Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
- 50X TAE (Tris-Acetate-EDTA) Buffer is used for running electrophoresis of nucleic acids in the gels for providing electrical conductivity and maintaining the pH
- Contains 2M Tris, 1M Acetic acid with 0.05M EDTA
- Lower buffering capacity than TBE buffer, hence periodic replacement is recommended during prolonged electrophoresis
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for DNA electrophoresis and purification processes facilitating accurate analyses in molecular biology experiments.
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Educational Institutions
Essential for teaching laboratories, providing students a hands-on understanding of nucleic acid separation techniques.
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Research Laboratories
Beneficial for academic and industry research labs performing extensive genetic research and cloning procedures.
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Non-scientific Users
Not suitable for casual or non-research users who lack the expertise in using laboratory reagents effectively.
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Small Scale Users
Not ideal for small experiments that require minimal volumes, as 500 mL may be excessive for limited use.
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Clinical Diagnostics
Not appropriate for clinical diagnostic applications where specific buffers are typically mandated for safety and accurate results.
Product Description
50X TAE Buffer (Tris-Acetate-EDTA), 500 mL
About This Item
Introducing 50X TAE Buffer Tris-Acetate-EDTA, 500mL, the perfect solution for your DNA electrophoresis experiments. This laboratory-grade buffer offers high purity and is ideal for research purposes, allowing you to achieve accurate and reliable results. Our Tris-Acetate-EDTA buffer contains 2M Tris, 1M Acetic acid with 0.05M EDTA, providing excellent electrical conductivity and maintaining the pH during electrophoresis.
The EDTA present in the buffer chelates divalent cations, inhibiting metal-dependent nucleases and protecting DNA from degradation during electrophoresis, for optimal results. Our Acetate-EDTA buffer may be used for both genomic and large supercoiled DNA, and as a gel preparation buffer. It is perfect for DNA fragment analysis, nucleic acid separation, DNA extraction, and genomic DNA isolation.
With a lower buffering capacity than TBE buffer, periodic replacement of the buffer during prolonged electrophoresis is recommended. However, dsDNA runs faster with TAE buffer than TBE. Our 50X concentrated TAE buffer should be diluted with molecular biology grade water to 1X before use.
It is easy to prepare and use, making it perfect for PCR analysis and gel electrophoresis applications. Choose our Molecular grade TAE buffer for reliable and accurate results in all your DNA electrophoresis experiments. Order now and experience the convenience and quality provided by 50X TAE Buffer Tris-Acetate-EDTA, 500mL.
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Customer Questions & Answers
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Question:
What is TAE Buffer and its purpose in molecular biology?
Answer: TAE Buffer, which stands for Tris-Acetate-EDTA, is a buffer solution commonly used in molecular biology, particularly in DNA and RNA electrophoresis. It helps maintain a stable pH during experiments, which is crucial for preserving nucleic acid integrity. By using TAE Buffer, researchers can effectively separate genetic material, making it an essential tool in laboratories for applications like gel electrophoresis and cloning. -
Question:
How should I store 50X TAE Buffer?
Answer: 50X TAE Buffer should be stored at room temperature or in a cool, dark place. Protecting it from light helps prevent degradation of the Tris component, ensuring its effectiveness. Using proper storage techniques prolongs the buffer's shelf life, enabling multiple experiments over an extended period without compromising results. -
Question:
Can I dilute 50X TAE Buffer for use in agarose gel electrophoresis?
Answer: Yes, 50X TAE Buffer should be diluted to 1X before use in agarose gel electrophoresis. To prepare a 1X solution, you can mix one part of the 50X stock with 49 parts of distilled water. This dilution ensures that the buffer maintains the correct pH and ionic strength required for effective separation of nucleic acids during electrophoresis. -
Question:
What are the advantages of using TAE Buffer over TBE Buffer?
Answer: TAE Buffer has several advantages over TBE Buffer, including lower conductivity, which allows for improved resolution of large DNA fragments. Additionally, TAE can be reused multiple times, making it cost-effective for researchers. Its composition also allows for better performance in applications where DNA integrity is critical, such as in Southern blotting or when preparing DNA for sequencing. -
Question:
Is 50X TAE Buffer suitable for RNA gel electrophoresis?
Answer: Yes, 50X TAE Buffer is suitable for RNA gel electrophoresis, as it provides a stable environment that helps preserve RNA integrity during electrophoretic separation. Unlike some other buffers, TAE does not chelate divalent cations that might destabilize RNA. This makes it a practical choice for visualizing RNA, studying gene expression, or analyzing RNA transcripts. -
Question:
What is the role of EDTA in TAE Buffer?
Answer: EDTA, or ethylenediaminetetraacetic acid, is included in TAE Buffer to chelate divalent metal ions such as magnesium and calcium. These metal ions can catalyze the degradation of nucleic acids; therefore, by binding to them, EDTA helps to protect DNA and RNA from enzymatic digestion, preserving their integrity during experiments. -
Question:
Who would typically use 50X TAE Buffer?
Answer: 50X TAE Buffer is mainly used by molecular biologists, genetic researchers, and educators in laboratory settings. It's invaluable for tasks like DNA isolation, restriction enzyme digestion analysis, and cloning experiments. Anyone involved in the study of genetics or forensic science would find this buffer vital in their research and practical applications. -
Question:
What safety precautions should I take when using 50X TAE Buffer?
Answer: When handling 50X TAE Buffer, it’s important to wear gloves and goggles to avoid skin and eye contact, as it can cause irritation. Ensure that your work area is well-ventilated and that any spills are cleaned up promptly. Additionally, following proper waste disposal methods for chemical products will help maintain a safe laboratory environment. -
Question:
Can 50X TAE Buffer be used for other applications besides electrophoresis?
Answer: Yes, 50X TAE Buffer can also be used in various applications beyond electrophoresis. It serves as a buffer for DNA ligation, restriction digestion, and PCR reactions, largely due to its ability to maintain a stable pH and ionic conditions. Its versatility makes it a go-to buffer for numerous molecular biology techniques. -
Question:
Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 500 mL in Tajikistan?
Answer: You can conveniently purchase 50X TAE Buffer Tris-Acetate-EDTA, 500 mL from Ubuy. Ubuy offers a reliable online platform with a wide selection of laboratory supplies, making it a great option for your research needs.
eConLabSupply Buffers Editorial Review
The 50X TAE Buffer Tris-Acetate-EDTA, 500mL is a reliable and efficient solution for DNA and RNA electrophoresis. It offers a high-quality, consistent performance that meets the needs of researchers and scientists. The buffer comes in a convenient 500mL bottle, providing ample supply for multiple experiments. The TAE buffer is easy to prepare and ensures precise and accurate results when used in gel electrophoresis. Overall, this product delivers exceptional value and is highly recommended for laboratory use.
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Pros
- Reliable and efficient solution for DNA and RNA electrophoresis
- High-quality and consistent performance
- Convenient 500mL bottle
- Easy to prepare
- Delivers exceptional value
Product Price History
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Features & Benefits
- Contains 2M Tris, 1M Acetic acid with 0.05M EDTA
- Inhibits metal dependent nucleases by chelating the divalent cations
- Protects DNA during electrophoresis
- Can be used for genomic and supercoiled DNA
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only, not for human or animal clinical/therapeutic use.