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97962882
10X TAE Buffer (Tris-Acetate-EDTA), 1L
товар №:
97962882
TJS 806
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Импортировано из магазина USA
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| Item Weight | 2.2 lbs (1 kg) |
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10X TAE Buffer (Tris-Acetate-EDTA), 1L
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Вопросы и ответы клиентов
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вопрос:
What is 10X TAE Buffer Tris-Acetate-EDTA used for?
отвечать: 10X TAE Buffer is primarily used in molecular biology applications for agarose gel electrophoresis. It functions as a running buffer, maintaining the stability of nucleic acids during the separation process. The Tris provides a stable pH, while acetate helps in the effective separation of DNA fragments based on size. It’s an essential reagent for researchers involved in DNA analysis, cloning, and various laboratory procedures where nucleic acid handling is required. -
вопрос:
How do you prepare 1X TAE Buffer from 10X TAE Buffer?
отвечать: To prepare a 1X TAE Buffer from a 10X concentration, simply dilute it by mixing one part of 10X TAE with nine parts of distilled water. For example, if you need 100 mL of 1X TAE, combine 10 mL of 10X TAE with 90 mL of distilled water. This diluted solution is essential for various applications in gel electrophoresis, ensuring that your DNA samples are adequately buffered during the analysis process. -
вопрос:
What are the components of 10X TAE Buffer?
отвечать: 10X TAE Buffer contains Tris (tris(hydroxymethyl)aminomethane), acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent to maintain a stable pH, acetate assists in the efficient migration of nucleic acids during electrophoresis, and EDTA chelates divalent metal ions, which helps protect DNA from degradation. Each component plays a critical role in ensuring successful separation and visualization of nucleic acids during laboratory experiments. -
вопрос:
Why is EDTA included in the 10X TAE Buffer formulation?
отвечать: EDTA is included in the 10X TAE Buffer as a chelating agent. It binds to metal ions such as magnesium and calcium that could potentially catalyze the degradation of nucleic acids. This protection is crucial when handling DNA samples, allowing for clearer results during electrophoresis. Researchers often use 10X TAE in procedures that involve sensitive DNA, making EDTA a vital component of this buffer solution. -
вопрос:
Can 10X TAE Buffer be used for RNA applications?
отвечать: While 10X TAE Buffer is primarily designed for DNA applications, it can also be used for RNA applications, particularly in gel electrophoresis. The buffer's components facilitate RNA stability, but additional care should be taken to use RNase-free reagents to prevent RNA degradation. Thus, 10X TAE can be effectively employed in experiments involving RNA, making it versatile for various nucleic acid studies. -
вопрос:
Is 10X TAE Buffer suitable for high-resolution gel electrophoresis?
отвечать: Yes, 10X TAE Buffer is suitable for high-resolution gel electrophoresis and is often preferred for DNA fragment separation. The buffer's pH stability and low ionic strength allow for the precise separation of smaller DNA fragments, making it ideal for applications like DNA fingerprinting and cloning. Researchers often choose it to achieve clearer bands in their agarose gel, helping in accurate analysis and visualization. -
вопрос:
What is the storage requirement for 10X TAE Buffer?
отвечать: 10X TAE Buffer should be stored at room temperature or in a refrigerator, away from light. Proper storage helps preserve the stability of its components, ensuring optimal performance during experiments. Checking for any precipitation or color changes before use is a good practice. By following these storage guidelines, researchers can ensure their buffer maintains its efficacy in future applications. -
вопрос:
Can 10X TAE Buffer be reused after gel electrophoresis?
отвечать: Generally, reusing 10X TAE Buffer after gel electrophoresis is not recommended. The buffer may become contaminated with nucleic acids or other components from the gel, which can alter its composition and affect subsequent experiments. For reliable results, it is best to use fresh buffer for each run, ensuring that sample integrity and separation accuracy are maintained throughout different experiments. -
вопрос:
What safety precautions should be taken when handling 10X TAE Buffer?
отвечать: When handling 10X TAE Buffer, it's important to wear appropriate personal protective equipment, such as gloves and goggles, to avoid contact with skin or eyes. Although the buffer is relatively safe to use, direct exposure to any chemical components could irritate. Always work in a well-ventilated area and ensure proper disposal practices for any unused buffer or waste solutions, adhering to your laboratory's safety protocols. -
вопрос:
Where can I buy 10X TAE Buffer Tris-Acetate-EDTA, 1L in Tajikistan?
отвечать: You can buy 10X TAE Buffer Tris-Acetate-EDTA, 1L from Ubuy, which offers a wide range of laboratory chemicals and supplies. Ubuy is known for providing quality products and can help you find the exact specifications you need for your research or educational projects, making it a reliable option for scientists and students alike.
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Плюсы
- Highly effective buffering agent
- Stable pH over time
- Great for gel electrophoresis
- Convenient 1L size
- Trusted by labs worldwide
Минусы
- May have a slight odor.
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Особенности и преимущества
- Comprehensive range of biological buffers for research, diagnostics, and drug development.
- Produced in an ISO certified facility ensuring high quality standards.
- 10X TAE Buffer provides optimal conditions for nucleic acid electrophoresis.
- EDTA in the buffer protects DNA from nucleases during electrophoresis.
- 10X TE Buffer is ideal for solubilizing DNA or RNA while preventing degradation.
- Buffers suitable for genomic and supercoiled DNA applications.