PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit
TJS 1526
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Apex Liquid Buffers are crafted using ultra pure or cGMP grade chemicals, ensuring high quality for all your molecular biology needs.
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| Item Weight | 1.1 lbs (500 grams) |
ОПИСАНИЕ ТОВАРА
PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit
Вопросы и ответы клиентов
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вопрос:
What is PBST (PBS-Tween 20) used for?
отвечать: PBST, or PBS-Tween 20, is commonly used in biological and biochemical research. It acts as a buffer solution that maintains a stable pH and is particularly effective in wash steps during cell and protein assays. The addition of Tween 20, a non-ionic detergent, reduces non-specific binding of proteins to surfaces, improving the clarity and accuracy of experimental results. This makes PBST particularly valuable in applications like ELISA, Western blotting, and immunohistochemistry. -
вопрос:
What are the components of PBST?
отвечать: PBST consists of phosphate-buffered saline (PBS) and Tween 20. PBS is an isotonic buffer containing sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate, which helps maintain osmotic balance. Tween 20 is a surfactant that helps reduce protein aggregation and facilitates better penetration in assays, leading to more reliable outcomes. Together, these components optimize conditions for various biological applications. -
вопрос:
How do you prepare PBST from the 10X stock solution?
отвечать: To prepare PBST from the 10X stock solution, simply dilute the stock solution in distilled water to reach a final concentration. For example, mix one part of the 10X PBST with nine parts of distilled water to achieve a 1X solution. This dilution ensures that the pH and buffer capacity of the solution are maintained, allowing for optimal performance in your assays. Always check the pH as a best practice to ensure it remains around 7.4. -
вопрос:
What is the significance of the pH 7.4 in PBST?
отвечать: The pH of 7.4 in PBST is critical as it closely matches the physiological pH of most biological systems. This neutral pH ensures that proteins and cells remain stable and functional during experimental processes. Maintaining this pH level can greatly influence the efficiency of antibody binding and protein interactions, making it vital in experiments such as immunoassays where biological relevance is key. -
вопрос:
Can PBST be stored for later use?
отвечать: Yes, PBST can be stored for future use, provided it is kept under appropriate conditions. Store the solution at 4°C to maintain its stability and effectiveness. However, it’s advisable to avoid repeated freeze-thaw cycles which can degrade the solution's components. When stored correctly, PBST can remain effective for several weeks, making it convenient for multiple experiments. -
вопрос:
Is PBST safe to use in a laboratory environment?
отвечать: PBST is generally considered safe for use in laboratory environments, with no known toxic effects from its components. However, like any reagent, it should be handled with care, using appropriate personal protective equipment, such as gloves and goggles. It is always good practice to review the safety data sheet (SDS) for proper handling guidelines to ensure a safe working environment. -
вопрос:
What are some common applications of PBST in research?
отвечать: PBST is widely used in various research applications, including ELISA assays for quantitative analysis of proteins and Western blotting for the detection of specific proteins. Moreover, it is beneficial in immunocytochemistry techniques to detect antigens in cells and tissues. Its role in these applications enhances the reliability and clarity of results, crucial for scientific studies. -
вопрос:
How does the presence of Tween 20 affect experimental outcomes?
отвечать: The inclusion of Tween 20 in PBST enhances experimental outcomes by minimizing non-specific binding and improving the accessibility of antibodies to their targets. This surfactant disperses proteins and helps maintain their stability, resulting in clearer assay results. Consequently, using PBST with Tween 20 can significantly enhance sensitivity and specificity in applications such as ELISA and Westerns. -
вопрос:
What is the shelf-life of PBST once opened?
отвечать: Once opened, PBST should ideally be used within a certain timeframe to ensure its effectiveness, typically within 2-4 weeks when stored at 4°C. The shelf-life can be affected by factors such as contamination or exposure to light. To maximize its usability, it is recommended to avoid touching the inside of the cap or the opening of the bottle, ensuring the integrity of the solution. -
вопрос:
Where can I buy PBST (PBS-Tween 20), 10X, pH 7.4, 500ml/Unit in Tajikistan?
отвечать: You can purchase PBST (PBS-Tween 20), 10X, pH 7.4, 500ml/Unit from Ubuy in Tajikistan. Ubuy offers a variety of laboratory reagents and supplies with the convenience of online shopping, ensuring you can easily find and acquire the essential products you need for your research.
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TJS 1526
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Особенности и преимущества
- Uses ultra pure or cGMP grade chemicals for maximum quality.
- Contains 4 fold purified water for enhanced performance.
- Ideal for molecular biology research and protein analysis.
- Purity exceeds 99%, ensuring reliable results.
- Free from RNase, DNase, endonucleases, endotoxins, proteins, and proteases.
- Perfect for purification, crystallization, and diagnostic applications.